iPSCs Reprogramming Tools

All the Tools You will Need for iPSCs Reprogramming - High Efficiency, Proven Quality and Broad Choices...

Introduction

The expression of the four human transcription factors (TFs) (Yamanaka Oct4, Klf4, Sox2, and c-Myc) or (Thomson: Oct4, Sox2, Nanog, Lin28) have been shown to reprogram different types of terminally differentiated cells to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cells (Yu, et al., 2007; Takahashi, et al., 2007). ABM Inc. has developed the most comprehensive product line for iPSC generation and related stem cell research areas. This includes iPSC purified recombinant proteins, minicircle DNA, iPSC lentiviral and adenoviral particles, EBV-based non-integrated plasmids, and piggyBac vectors. With those tools available, you can generate iPSC from any cell type with choices of both transient (proteins, adenovirus, and plasmids) and stable (lentivirus) iPSC factors. In addition, choices of iPSC factors driven by different promoters in lentivirus (CMV, UBC, PGK, and EF1α) are also available.

Stringent Quality Testing

ABM’s application testing ensures the highest quality of reprogramming recombinant proteins, lentiviruses, adenoviruses, and plasmids. Every lot of products have been confirmed to successfully produce iPSCs or to express specific iPSC factors in cell types. With proven quality, you can minimize the need to repeat experiments and advance your project efficiently.

Choices for Different Formats, Concentrations, and Species

ABM’s broad iPSC product line enables researchers to select the right product applicable to their research – no need to compromise. Products are provided in the formats of recombinant proteins, viral particles, and plasmids for both human and mouse iPSC factors. Custom development of any specialized iPSC products is also available. All known six reprogramming factors are available as individual viruses and in functional sets.

Choose Your iPSC Product Choice

Click on the main product title to download product brochure for more information.

Minicircle & Plasmids

The only Minicircle with an EF1α promoter, a truly xeno-free plasmid without any sequence of bacterial or viral origin. Generation of vector-free iPSCs via oriP/EBNA1 (Epstein-Barr nuclear antigen-1)-based episomal vectors and piggyBac transposon-based plasmids.

Viral Particles (Lenti/Adenoviruses)

Most comprehensive iPSC reprogramming lentiviruses including all 6 iPSC genes (both mouse and human) with choices of EF1α, PGK, UBC, and CMV promoters. Available in pre-made ready to ship Lenti/Adenoviruses.

Recombinant Proteins

Eliminates possible genome mutations and insertions associated with DNA transfection or viral transduction by choosing recombinant proteins instead. Crude lysates/Purified iPSCs recombinant proteins available in sets or as individual factors.

Small Molecules & Growth Factors

Substitute and regulate genetic function of iPSCs transcription factors with defined small chemical molecules. All small molecules are of the highest purity (≥98%), structurally verified by NMR and mass spectrometry, and tested for cytotoxicity. iPSCs growth factors allow you to perform reproducible results in all your stem cell projects without any interference from other protein contaminants.

Antibodies

Wide selection of antibodies for iPSC and stem cell research, and all ABM's antibodies are well-characterized for the applications indicated.

iPS Cell Lines

ABM provides licenced iPSCs of different origins and induction method from institutes around the world.

Characterization Tools

Experience fast and effective characterization with ABM's Human Tri-Lineage Multiplex PCR Kit. Evaluate transduction of target cells with ABM's Stemporter.

iPS Custom Services

iPSC generation is a complex cellular process, which is time-consuming and technically challenging. Scientists at ABM have developed streamed-line protocols for efficient iPSC generation with plasmid, viral vectors, and recombinant proteins. By outsourcing iPSC service to ABM, you will save time and money for your ongoing project. Contact us for more information.

To order, simply drop us an email at sales@Bio-REV.com or contact us at (65) 6273 3022.