BIO-REV

iPSCs Reprogramming Tools

katie — Mon, 13 Jun 2011 04:38:53 +0000

All the Tools You will Need for iPSCs Reprogramming – High Efficiency, Proven Quality and Broad Choices…

Introduction

The expression of the four human transcription factors (TFs) (Yamanaka Oct4, Klf4, Sox2, and c-Myc) or (Thomson: Oct4, Sox2, Nanog, Lin28) have been shown to reprogram different types of terminally differentiated cells to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cells (Yu, et al., 2007; Takahashi, et al., 2007). ABM Inc. has developed the most comprehensive product line for iPSC generation and related stem cell research areas. This includes iPSC purified recombinant proteins, minicircle DNA, iPSC lentiviral and adenoviral particles, EBV-based non-integrated plasmids, and piggyBac vectors. With those tools available, you can generate iPSC from any cell type with choices of both transient (proteins, adenovirus, and plasmids) and stable (lentivirus) iPSC factors. In addition, choices of iPSC factors driven by different promoters in lentivirus (CMV, UBC, PGK, and EF1α) are also available.

Stringent Quality Testing

ABM’s application testing ensures the highest quality of reprogramming recombinant proteins, lentiviruses, adenoviruses, and plasmids. Every lot of products have been confirmed to successfully produce iPSCs or to express specific iPSC factors in cell types. With proven quality, you can minimize the need to repeat experiments and advance your project efficiently.

Choices for Different Formats, Concentrations, and Species

ABM’s broad iPSC product line enables researchers to select the right product applicable to their research – no need to compromise. Products are provided in the formats of recombinant proteins, viral particles, and plasmids for both human and mouse iPSC factors. Custom development of any specialized iPSC products is also available. All known six reprogramming factors are available as individual viruses and in functional sets.

Choose Your iPSC Product Choice

Click on the main product title to download product brochure for more information.

Minicircle & Plasmids

The only Minicircle with an EF1α promoter, a truly xeno-free plasmid without any sequence of bacterial or viral origin. Generation of vector-free iPSCs via oriP/EBNA1 (Epstein-Barr nuclear antigen-1)-based episomal vectors and piggyBac transposon-based plasmids.

Viral Particles (Lenti/Adenoviruses)

Most comprehensive iPSC reprogramming lentiviruses including all 6 iPSC genes (both mouse and human) with choices of EF1α, PGK, UBC, and CMV promoters. Available in pre-made ready to ship Lenti/Adenoviruses.

Recombinant Proteins

Eliminates possible genome mutations and insertions associated with DNA transfection or viral transduction by choosing recombinant proteins instead. Crude lysates/Purified iPSCs recombinant proteins available in sets or as individual factors.

Small Molecules & Growth Factors

Substitute and regulate genetic function of iPSCs transcription factors with defined small chemical molecules. All small molecules are of the highest purity (≥98%), structurally verified by NMR and mass spectrometry, and tested for cytotoxicity. iPSCs growth factors allow you to perform reproducible results in all your stem cell projects without any interference from other protein contaminants.

Antibodies

Wide selection of antibodies for iPSC and stem cell research, and all ABM’s antibodies are well-characterized for the applications indicated.

iPS Cell Lines

ABM provides licenced iPSCs of different origins and induction method from institutes around the world.

Characterization Tools

Experience fast and effective characterization with ABM’s Human Tri-Lineage Multiplex PCR Kit. Evaluate transduction of target cells with ABM’s Stemporter.

iPS Custom Services

iPSC generation is a complex cellular process, which is time-consuming and technically challenging. Scientists at ABM have developed streamed-line protocols for efficient iPSC generation with plasmid, viral vectors, and recombinant proteins. By outsourcing iPSC service to ABM, you will save time and money for your ongoing project. Contact us for more information.

To order, simply drop us an email at sales@Bio-REV.com or contact us at (65) 6273 3022.

Bioneer siRNAs FAQs

katie — Thu, 21 Apr 2011 05:51:15 +0000

Synthesis and Order

Q1. How is siRNA synthesized?

The most commonly used method in siRNA synthesizers is the ‘phosphite triester’ method, developed by Koster. This method builds the phosphodiester backbone of the RNA molecule by using β-cyanoethyl phosphoramidites. (Nucl. Acids Res. 1984, 12, 4539 ; Tetrahedron Lett. 1983, 24,5843) The synthesis begins with a solid support (CPG) with an attached starter nucleoside. A deblocking-coupling-capping-oxidation cycle is repeated to reach the desired length of siRNA molecule. After synthesis, ammonia is used to detach the siRNA from the solid support. The siRNA then undergoes deprotection and TBDMS treatment before being purified. The end result of these processes is pure siRNA. Each synthesized ss-RNA is annealed to produce the final duplexed siRNA form.

Q2. How do I order pre-designed siRNAs?

The AccuTarget Genome-wide Predesigned siRNAs are designed for Human, Mouse and Rat genomes. You may order pre-designed siRNAs through Bio-REV. The desired target can be searched by Symbol, Gene No, Refseq accession Number or Description. Each query will yield three (3) candidates with the highest predicted efficiency score. You may select the desired quantity at that point. Also, you may select purification type and the guaranteed nmole quantity as well.

Q3. When can I expect delivery?

Delivery depends on the AccuTarget product that is ordered. Custom siRNA orders can be made and delivered in 10 business days. AccuTarget Predesigned siRNA can be delivered within 5 days of order receipt. Library products, orders of < 10 96-well plates can be shipped within 3 days of order receipt, and shipping dates for higher quantities above that will have to be discussed on a case-by-case basis.

Q4. Up to how many bases can Bioneer synthesize?

The maximum length of a ss-RNA molecule is 35 bases. We charge per base for this type of order. For siRNA molecules, the maximum length is 30 bases at a flat price.

Q5. What form will my order be in?

For Genome-Wide Predesigned siRNAs, Validated siRNAs, siRNA Libraries and Control siRNAs, both the sense and antisense strands are synthesized at equimolar concentrations, verified via MALDI-TOF, then annealed and delivered in duplexed, lyophilized form. You may reconstitute the siRNA with a buffer of your choice or with ultrapure water that we provide with every order. We recommend reconstituting to 100 μM. For Custom siRNA orders, the order is processed by the same method as above. We recommend 50 μM reconstitution for Custom siRNA orders. When ordering Custom siRNA you must select the “Annealing Service” to receive your order in annealed form. If you choose not to use our annealing service, you can use 1X annealing buffer and follow the annealing protocol included with your Custom siRNA order.

Q6. I want to conduct an in vitro experiment. What scale should I choose? What purification should I select?

With a 10 nmole scale siRNA order, you can transfect one hundred (100) 96-well plates at 100 nM per transfection. Unless you are planning to conduct an in vivo experiment, the Bio-RP Purification will yield outstanding results. We recommend HPLC purification for in vivo experimental use.

Q7. Can Bioneer synthesize chimeric RNA?

Yes we can. We have the ability to synthesize chimeric RNA molecules that incorporate dA, dC, dG, and dT DNA bases. We can also incorporate 2′-O-Methyl and 2′-F(rU, rC) into the RNA. Because these are special orders, you must contact Bio-REV via email (sales@bio-REV.com) or call us at (65) 6273 3022 so that we may fulfill the request to your satisfaction.

Q8. I would like to order over four (4) siRNA candidates for a single gene. How do I order?

Our Genome-wide predesigned siRNAs provide three (3) candidates per target gene. In order to request more than four (4) candidates, order via Custom siRNA request. The siRNA sequences can be verified only after the order has been submitted. If you would like to compare the sequences with publications or to modify your order, please email us at sales@bio-REV.com or call us at (65) 6273 3022.

Q9. The Genome-wide predesigned siRNA didn’t work like I expected. What do I do?

Q10. Are phosphate groups present on the 5′ or 3′ ends of the synthesized siRNA?

Unless explicitly stated, the 5′ and 3′ ends are capped with -OH groups. Therefore, to order 5′ phosphate-capped siRNAs, you must request for 5′ phosphorylation modification.

Modified siRNA

Q1. What types of modified siRNAs are there?

In order to maximize siRNA applications, modified* siRNAs are available. Although there are many ways to modify siRNAs, the most convenient method is to use phosphoramidites to label siRNAs during synthesis. For a 5′ siRNA modification, synthesis is first conducted as normal. At the final stage, however, a labeling phosphoramidite is attached to the 5′ end, resulting in a 5′ labeled siRNA. 3′ labeling is somewhat complicated in that we must start with a labeled phosphoramidite attached to a solid support and build the molecule from there. You can incorporate the label of your choice to the siRNA sequence by using a labeled phosphoramidite at the 5′ end of deoxyuridine, therefore allowing for an internal modified siRNA.

The following modifications are offered for our siRNAs:

5′ Amine – 3′ amine modification
5′ Phosphorylation & 3′ phosphorylation
5′ Thiol – 3′ Thiol Modification
5′ Biotin – Internal Biotin-dT Modification
5′ Fluorescein – 3′ Fluorescein modification
3′ TAMRA modification

*Certain items may not be available in all countries.

Handling and Storage

Q1. How to I store my siRNAs and how long do they keep?

siRNAs can normally be kept stable at -20°C for over 1 year. The lyophilized form is especially stable and has a long shelf-life. Although solution-dissolved siRNAs can be stable, contamination of the reconstitution solution with RNase will degrade the product. Also, repeated freeze-thaw cycles accelerate the degradation process. Therefore, we recommend that after you receive the siRNA stock, you reconstitute and make several aliquots to avoid such freeze-thawing. Because the phosphodiester bonds of the RNA can break under high pH conditions, we ask you to take caution, and recommend reconstituting in ultrapure water provided.

Q2. How do I reconstitute my siRNA?

If you would like to keep your siRNA in solution, we recommend reconstituting with DEPC-treated water that we provide with your order to maximize stability.

Q3. How to I store my fluorescent dye modified siRNA?

Photobleaching may occur if the fluorescent dye modified siRNA is exposed to light for prolonged periods of time. Therefore we recommend that you store such siRNAs in a dark container and store that container in a dark place.

Purification

Q1. I would like to know how the synthesized siRNA is purified.

To purify newly synthesized siRNA, we have several methods including Bio-RP, HPLC and PAGE. We select a purification method depending on the end-use of the product:

Bio-RP: Phosphoramidites used for siRNA synthesized with the Bio-RP synthesizer have a DMT group protecting the 5′ end to prevent accessory reactions from occurring during synthesis. If the DMT group is not removed at the end of the synthesis cycle (‘trityl-on’ mode), only the desired length N-mer siRNA will have the 5′-DMT group, while the shorter N-1, N-2 etc. synthesis by-products will not have a DMT group attached. Because the DMT group is extremely hydrophobic, it will interface very well with the RP resin. By taking advantage of this fact, and the fact that DMT groups are not present in the N-1, N-2 etc. by-products, we are able to efficiently purify the desired N-mer siRNA. Unless intended for in vivo experiments, the Bio-RP Purification method will yield excellent experimental results.
HPLC: For experiments requiring extremely pure siRNAs such as in vivo experiments, Bio-RP purification may be insufficiently pure. In this case, HPLC is commonly used to reach the desired purity level. We use a reversed-phase resin for purification, and this will routinely yield up to 98% purity for 21-mer siRNA molecules. We recommend selecting HPLC purification if you are intending to use the siRNA for in vivo experiment use.

Quantification and Concentration

Q1. What does the O.D. value mean?

Because the amount of synthesized siRNA is extremely small, it is very difficult to quantify by weight. Therefore, we capitalize on the light-absorption properties of nucleic acids to indirectly measure the amount of product. The amount of light absorbed by a given amount of product is expressed as an O.D. (Optical Density) value, and is directly related to the amount of product in solution.

Q2. How do I calculate the amount of siRNA from the O.D. value?

After measuring the amount of 260 nm light absorbed by the siRNA, the following formula is used to calculate the actual amount of siRNA O.D. = εC. The ε is the extinction coefficient which is unique for different materials, and the C stands for the siRNA concentration. If one knows the extinction coefficient and the O.D. value of the siRNA, the concentration can be calculated by substituting those values in the formula above. The ε value for an siRNA molecule is the sum of the extinction coefficient of each base constituting the molecule. The extinction coefficients for each base at 260 nm is are as follows:

rG : 11.5 ml/μmole
rC : 7.2 ml/μmole
rA : 15.4 ml/μmole
rU : 9.9 ml/μmole

Therefore, the extinction coefficient for the entire siRNA molecule can be calculated by multiplying the number of bases by the corresponding extinction coefficient and adding all four products together.

Q3. If my 18-mer siRNA (3G, 4C, 5A, 6U) O.D. value was 0.7, then how much siRNA do I have?

Let’s start by calculating the extinction coefficient (εε) of the entire siRNA molecule. ε= 11.5 x 3 + 7.2 x 4 + 15.4 x 5 + 9.9 x 6 = 199.7 (ml/μmole) Therefore, using the O.D. = εC formula, we can calculate the final concentration C to be C = 0.7 /199.7 = 0.0035 (μmole/ml) = 3.5 (nmole/ml). This value is expressed as the ‘total nmole’ value in your copy of the oligo synthesis report.

Q4. How do I calculate the molecular weight of the synthesized siRNA molecule?

Substitute the number of each base into the following formula: M.W. = (NA x 329.208) + (NC x 305.183) + (NG x 345.207) + (NU x 306.168) – 63.98) + 2.016 NA = Total number of rA NC = Total number of rC NG = Total number of rG NU = Total number of rU

Q5. Can I know how many ng the synthesized product is?

Normally, we will fulfill an order with a guaranteed nmole amount, and the synthesis report will also report the final amount in nmoles. If you must have the ng amount to calculate for an experiment, you can convert from nmole to ng by using the formula below. We make it easy for you by giving you the molecular weight of the siRNA sequence in the report. Molecular Weight (g) X mole (nmole) = Mass of siRNA (ng)

Q6. How do I reach a target concentration?

Each synthesis report gives you a ‘volume for 100 pmole/μl’ value that stands for the volume of DEPC-treated water you need to add to achieve a 100 pmole/μl concentration. As an example, if the ‘volume for 100 pmole/μl’ values for two siRNAs are 100 and 200, then you can reconstitute with 100μl and 200 μl of DEPC-treated water, respectively, to reach a 100 pmole/μl concentration.

In other words, the siRNA contained in each tube is:
100 μl x 100 pmole/μl = 10,000 pmole = 10 nmole,
200 μl x 100 pmole/μl = 20,000 pmole = 20 nmole
Although the final use will dictate the concentration of siRNA, we found that for average use, 100 pmole/μl is ideal. That is why we provide you with DEPC-treated water volume for reconstitution to 100 pmole/μl.

Q7. How do I convert between morlarity and moles?

The standard concentration units for oligomers is given in M (mole/L), and the prefixes such as μ (micro), n (nano), p (pico) etc. describe the scope of the unit. The following prefixes are used not only for M, but for other measurement units such as length, mass etc.

10^-1 = deci [d] 10¹ = deca [da]
10^-2 = centi [c] 10² = hecto [h]
10^-3 = milli [m] 10³ = kilo [k]
10^-6 = micro [μ] 10⁶ = mega [M]
10^-9 = nano [n] 10⁹ = giga [G]
10^-12 = pico [p] 10¹² = tera [T]
10^-15 = femto [f] 10¹⁵ = peta [P]
10^-18 = atto [a] 10¹⁸ = exa [E]
10^-21 = zepto [z] 10²¹ = zetta [Z]
10^-24 = yocto [y] 10²⁴ = yotta [Y]

1 pmole/μl
= 1×10-12 mole / 1×10-6 L
= 1×10-6 mole/L
= 1 μmole/L
= 1 μM

Therefore, μM and pmole/μl are one and the same.

Experiments

Q1. What are some precautions for a siRNA experiment?

First, because not all siRNAs will knock-down the target gene with identical efficiency, you should try 2-3 different sequences to find the best siRNA. Second, to make sure that the knock-down affects downstream protein expression, mRNA levels should also be measured. Thirdly, verify the knock-down phenotype by using another siRNA designed for the same target gene and show that the same phenotype appears.

Q2. This is my first siRNA experiment. How do I set my experimental conditions?

One of the most important factors in a siRNA experiment is the assessment of whether the siRNA gets delivered into the cell. Bioneer offers positive controls that can easily indicate whether the siRNA is being delivered successfully.

Q3. To what cell density should I culture my cells before siRNA transfection?

For siRNA transfection, we recommend that the cell density be approximately 60-70%. (HeLa cell, 6-well plate standard: 1.5 x 10⁵ cells/well. We also strongly encourage user optimization of these figures)

Q4. What concentration of siRNA should I use for transfection?

We recommend a starting concentration of 100 nM (100 pmol/well), but strongly advise empirical optimization for the cell line and conditions of your experiment.

Q5. How do I use 10 nmole of siRNA to transfect cells at 100 nM?

This is a source of confusion for many people. In order to transfect a single well with 100 nM siRNA where the transfection volume is 1 ml, you need 100pmole of siRNA. 2 µl of 50 µM (50 pmole/μl) stock siRNA solution in 1 ml will yield 100 pmole. If you were to order 10 nmole of a siRNA, it will be sufficient to transfect 100 wells at 100nM (100 pmole/ml).

Q6. What transfection reagent do I use?

Because each cell line will have different transfection efficiencies for every transfection agent, we recommend that you select the transfection reagent most suitable for your cell line.

Q7. How do I verify my siRNA transfection efficiency?

You can easily verify the transfection efficiency by transfecting your cells with NC-FITC and observing the cells with a fluorescence microscope. The NC-FITC can also be used as a test reagent to optimize the transfection concentrations of both the siRNA and the transfection reagent.

Q8. Is there a way to know my siRNA\’s knockdown efficiency beforehand?

The predicted siRNA knockdown efficiency can be seen at the target gene search screen by clicking the siRNA Number. Validated siRNA efficiencies will be presented in blue, while predesigned siRNA efficiencies will be displayed in grey.

Q9. What controls are used for a siRNA experiment?

The AccuTarget Negative Control siRNA is a non-targeting siRNA that has low sequence homology to all known Human, Rat and Mouse sequences. Therefore, it can be used as a convenient negative control for all Human, Rat and Mouse siRNA experiments. The AccuTarget™ Positive Control siRNAs (human) shows great knockdown efficiency for the target gene. We target GAPDH as an endogenous control gene, and also have positive control siRNAs for reporter systems such as GFP and Luciferase.

Q10. How do I verify the siRNA knockdown efficiency?

The siRNA knockdown efficiency can be verified through various techniques including qPCR, Northern Blot, Western Blot etc.

Q11. What are AccuTarget Genome-wide Predesigned siRNAs?

These siRNAs were predesigned for your convenience using our Turbo si-Designer, a siRNA design algorithm co-developed by us and KRIBB (Korea Research Institute of Bioscience & Biotechnology). Using this revolutionary algorithm, we have constructed a high-knockdown efficiency AccuTarget Genome-wide Predesigned siRNA database. As of this writing, our website contains predesigned siRNAs for Human (17,842 genes), Mouse (17,117 genes), and Rat (9,392 genes).

Q12. What are AccuTarget validated siRNAs?

After synthesizing siRNAs using our Turbo si-Designer, a siRNA design algorithm co-developed by us and KRIBB (Korea Research Institute of Bioscience & Biotechnology), AccuTarget Validated siRNAs have been measured for knockdown efficiency using qPCR. We verified that each siRNA shows at least a 70% knockdown efficiency. AccuTarget Validated siRNAs can be ordered with their corresponding real-time qPCR primer sets. Also, if you order 10 or more siRNAs, they will be delivered in flexible siRNA Library (tube or plate type) format.

Q13. Can I order siRNAs targeted for a specific gene?

You may order siRNAs for the target gene of your choice by entering the gene name or Accession number at our website and selecting one of the listed siRNA candidates. You may also order custom designed siRNA if your model organism is not Human, Mouse or Rat.

Q14. What are precautions for handling siRNA?

Wear gloves and a mask when handling siRNA.
Always use RNase free tubes and pipette tips.
Only use DEPC treated water when diluting siRNA.
Store fluorescence-labeled siRNA in a dark location.

Back to Product Description…

Bioneer AccuTarget siRNAs

katie — Wed, 20 Apr 2011 16:46:51 +0000

Turbo si-Designer: Highly effective in selecting functional siRNAs: 83.8% of the test siRNAs showed >75% knockdown and 38.1% elicited & 90% knockdown…

Successful siRNA experiments in mammalian cultured cells depend upon several factors. Specifically it is important to design and identify effective and specific siRNA sites and to perform efficient and specific delivery of siRNA to the desired target cell types. To facilitate design process, Bioneer, in collaboration with National Genome Information Center (NGIC) has developed Turbo siRNA designer, a proprietary siRNA selection algorithm.

Turbo si-Designer can identify highly effective siRNA target sites with superior success rates. The performance of the Turbo si-Designer was evaluated by designing hundreds of siRNAs and testing their knockdown efficacy by Real-Time PCR analysis. When compared with other web-based design tools, Turbo si-Designer algorithm successfully predicted functional siRNAs at high probability of efficient knockdown. Notably, siRNAs with the low score were mostly nonfunctional, indicating that ineffective siRNAs are efficiently removed by Turbo si-Designer (Fig. 1).

Bioneer manufactures high quality and cost-effective siRNA. Every siRNA is produced in an automated high-throughput RNA production system under clean room conditions and undergo rigorous QC tests.

Features and Benefits

Performance Guarantee:	When purchasing 3 siRNAs for the same gene, Bioneer guarantees at least an 80% reduction* in the target mRNA level for 2 of the three siRNAs.
Purification:	For your more demanding applications, Bioneer’s automated HPLC and Bio-RP purification methods ensure high quality, high- throughput siRNAs at an affordable price.
Affordable pricing:	Bioneer provides a variety of high quality siRNA products at an affordable price.
Synthesis and QC:	Bioneer siRNAs are produced in clean room facility by fully automated high-throughput siRNA production system. Bioneer siRNA products are assessed by MALDI-TOF Mass spectrometry analysis. Mass spec data is provided with every siRNA. Additionally, siRNAs are tested by gel electrophoresis to verify that both RNA strands annealed properly.

All Bioneer siRNAs are provided as double-stranded siRNA. Each sense siRNA and an antisense RNA are QC’ed by MALDI-TOF. Every annealed siRNA is then QC-tested using PAGE to confirm proper annealing.

Figure 1. MALDI-TOF mass spectrometry analysis of a custom siRNA. All siRNAs are processed by MALDI-TOF mass spectrometry to ensure its quality.

Figure 2. PAGE data of double-stranded custom siRNA. Complementary single-stranded RNA strands were hybridized to form siRNA duplex and analyzed by 15% non-denaturing PAGE.
SS: single-stranded RNA
DS: double-stranded siRNA

Figure 3. Confocal microscopic image of HeLa cells transfected with FITC-labeled negative control siRNA (Cat No.: SN-1021). The fluorescent cells indicate that the HeLa cells were successfully transfected with the siRNA.

Figure 4. Effects of Human GAPDH Positive Control siRNA. HeLa cells were transfected separately with AccuTarget Human GAPDH Positive Control and Negative Control siRNA using Lipofectamine 2000 (Invitrogen) at a final concentration of 100 nM. Total cellular RNA was isolated from cells 24 hours after transfection and subjected to Northern blot and Real-Time PCR analysis. As can be seen from Fig. 1-B, about 3% GAPDH mRNA remained.

Bioneer’s core business has always been the manufacture of DNA and RNA in conventional and breakthrough ways. When RNA interference (RNAi) was discovered, Bioneer was there to help provide researchers with the tools they need to accomplish their experiments. Bioneer is currently the market leader in RNAi technology in Asia, and is launching in North America this year. Our RNAi portfolio consists of six areas:

All Bioneer’s siRNAs are designed using our Turbo si-Designer software. This software identifies highly effective siRNA target sites with remarkably high knockdown rates. Critical parameters including base composition, thermodynamic instability and base preference are all considered in the design algorithm. siRNAs spanning SNP sites are removed and finally, non-specific siRNAs are eliminated following homology searching by BLAST and Smith-Waterman algorithms. The result is an siRNA with extraordinary knockdown efficiency and minimal off-target effects. We are so confident in our software that we have the best guarantee in the industry.

Notice to Purchaser

All siRNA Products: For Research Use Only. Not For Use in Diagnostic Procedures.

Limited License

This product is licensed under European Patents 1144623, 121945 and foreign equivalents from Alnylam Pharmaceuticals, Inc., Cambridge, USA and is provided only for use in academic and commercial research whose purpose is to elucidate gene function, including research to validate potential gene products and pathways for drug discovery and development and to screen non-siRNA based compounds (but excluding the evaluation or characterization of this product as the potential basis for a siRNA-based drug) and not for any other commercial purposes. Information about licenses for commercial use (including discovery and development of siRNA-based drugs) is available from Alnylam Pharmaceuticals, Inc., 300 Third Street, Cambridge, MA 02142, USA.

This product is sold for research use only and is not to be administered to humans or used for medical diagnostics. Buyer acknowledges and agrees that all intellectual property rights in the products (including, without limitation, the siRNA sequences used to create such products) and in any Bioneer technology, intellectual property and know-how used to make or useful for the manufacture or use of the products will at all times remain vested in Bioneer (other than any ownership interest that buyer may have in non-public proprietary target genes supplied by buyer to Bioneer in connection with custom products).

Trademark: AccuTarget is a trademark of Bioneer Corporation.

Product Brochure | FAQs

Realgene

katie — Wed, 20 Apr 2011 03:43:05 +0000

Realgene Bio-Technologies Inc. is located in Shanghai, China and is a bio-company specializing in providing bio-technical services including gene synthesis, peptide synthesis, DNA sequencing, cloning, mutagenesis, etc. and supplying reagents such as Pfu enzymen, Hotstart Taq Polymerase, dNTPs, etc. as well as labwares.

With a team of highly motivated biologist staffs and modern equipment and advanced precision apparatus including the real time PCR amplifier, polypeptide synthesizer, DNA synthesizer, DNA array analyzer, FPLC, etc., the have already successfully provided technical services including gene synthesis to a lot of customers throughout China, US and EU since 2003.

Based on the “land of treasure”, Realgene Bio-Technologies Inc. is committed to develop itself to the world’s leading manufacturer/provider of bio-products and services with the best quality and the most competitive-edge prices by making full use of the advantages in China.

Biologix

katie — Wed, 20 Apr 2011 03:31:37 +0000

Biologix Research Company is a U.S. Company founded in the year 2000 by Dr. Winston Lee, specializing in the manufacturing and supplying of plastic laboratory consumables.

They aim to deliver superior products, unbeatable prices, and excellent service to the scientific research community. Biologix is headquartered in the United States and all the production facilities are located in Mainland China. This strategic arrangement has made it possible for Biologix to utilize the latest technological breakthroughs to offer the life science communities less expensive and more efficient supplies for scientific research.

Biologix serves thousands of laboratories across the United States and have served distributors in countries all around the world. They are determined to provide customers just what they are looking for by engaging in market research, listening to our customer feedback, and participating in continuous product development.

Customer service is the one of the top priorities in their U.S. Headquarter office. Numerous phone calls are answered daily by their customer service representatives. Whether it be answering product questions, taking orders, or educating future distributors about our company and products, they are always happy to answer your questions.

NutriStem References

katie — Sun, 23 Jan 2011 14:31:33 +0000

James J. Collins, Chad Cowan, Thorsten M. Schlaeger and Derrick J. Rossi. Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA, Cell Stem Cell 7 (5): 618-630 (2010).
Human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells Shigeki Sugii, Yasuyuki Kida, Teruhisa Kawamura, Jotaro Suzuki, Rita Vassena, Yun-Qiang Yin, Margaret K. Lutz, W. Travis Berggren, Juan Carlos Izpisúa Belmonte, and Ronald M. Evans. (Sent for review September 3, 2009).
Corning Life Sciences Inc. introduces The Corning^® Synthemax™ Surface: A Synthetic, Xeno-Free Surface for Long-Term Self-Renewal of Human Embryonic Stem Cells in Defined Media, in 2010 world stem cell summit: ”Self-renewal and pluripotency of multiple hESC lines (H7, H1, H9, and BG01v) cultured on Synthemax Surface was compared to cells grown on Matrigel control surfaces under various defined medium conditions (StemPro^®, NutriStem™, mTeSR^®1, and XVIVO™ 10).” Click HERE.
The Hamilton Company and Global Cell Solutions developed a novel alginate microcarrier cell culture system that streamlines the cell culture process and enables efficient scale up of hESCs culture. When presenting the results on the use of this microcarriars suspension culture system to propagate undifferentiated hESC , Hamilton acknowledges NutriStem™ as one of the Newly developed “commercially available, partially or completely defined feeder free culture systems” along with: mTeSR1 (Stem Cell Tech.) and StemPro hESC SFM (Invitrogen)”. Click HERE and HERE.
Primorigen Biosciences introduces its StemAdhere™ Pluripotent Cell Growth Kit, representing images collected during ongoing comparative growth rate experiments between iPS cells grown on StemAdhere™ and Matrigel™ using three different media: NutriStem™, mTeSR^®1, and TeSR™2. The data suggest comparable growth rates of iPS cells between StemAdhere™ and Matrigel™ across all three media. Click HERE.

Back to Product Description…

Primary Cells

katie — Wed, 19 Jan 2011 02:18:56 +0000

Primary Cell Solutions^TM * Endothelial Cells * Fibroblasts * Keratinocytes * Melanocyte * Smooth Muscle Cell * Renal Epithelial Cell * Bronchial/Tracheal Epithelial Cells * Small Airway Epithelial Cell * Prostate Epithelial Cell * Mesenchymal Stem Cells * Microvascular Endothelial Cell * Corneal Epithelial Cell * Pre-Screened Cells

Human & Animal Primary Cells Lines * Endothelial Cells * Smooth Muscle Cells * Epithelial Cells * Progenitor Cells * Keratinocytes * Hepatocytes * Cardiomyocytes * Fibroblasts * Blood Cells * Mammary Cells * Microvascular Cells * Skin Cells * Brain Cells

Progenitor Cell Lines * Epidermis * Airway * Cornea * Prostate * Oral Mucosa * Bladder Epithelium * Mammary Epithelium * Vaginal Epithelium * Primary Cells Lines (Keratinocytes, Corneal/Gingival/ Bladder/ Dermal Epithelium)* Long Term Cell Models (Mouse Epidermal, Rat Airway/Prostate/Bladder/Vagina, Dermal Fibroblast)

LIFEBANK^TM Cellular System * Progenitor Cell Lines * Neural/Cardiovascular/Integumentary/Digestive/Pulmonary/Skeletal Muscles/Reproductive System * Pre-natal & Post-natal Cell Lines

Primary Cell Line Acquisitions * Customised Services

Clinical Diagnostic Tools

katie — Thu, 13 Jan 2011 09:37:10 +0000

Signature^® LTx Leukemia Translocation Panel v2.0 (RUO)* Signature^® NPM1 Mutations (RUO) *BCR/ABL1 Quant (RUO) * AmplideX™ FMR1 * Signature^® KRAS/BRAF (RUO) * Armored Controls * Custom Reagents

Molecular Diagnostics Tools (AccuPower Real-Time PCR Diagnostic Kits – STD / Hepatitis / Pathogen transmission in respiratory passages / Pathogen infection in organ transplantation / Genetic Diseases / Pre-natal or Paediatric Diseases / Gastro-intestinal or food-borne Diseases) * Influenza A ( H1N1 ) RT-PCR Diagnostic Kit

In vitro Diagnostics & Assays Kits & Reagents * Antigens & Antibodies for Diagnostic Assays (Oncology, Infectious Diseases, Skeletal System, Cardiovascular & Renal Systems, Gastroenterology & Nutrition, Clinical Immunology, Matrix Proteins) * ELISA Kits

Oligo & Gene Synthesis

katie — Thu, 13 Jan 2011 09:34:22 +0000

Standard & Modified DNA (AccuOliogo^®) & RNA Oligonucleotides * Custom DNA & RNA Oligonucleotides * High Throughput Oligonucleotides (HT-Oligo^TM) * Pre-made Primers * Custom Gene Synthesis

Antibodies

katie — Thu, 13 Jan 2011 09:31:05 +0000

Secondary Antibodies * Conjugates * Tag Antibodies

Monoclonal & Polyclonal Antibodies (AMPK, Akt, MAPK, mTor, Apoptosis, & Cytokine Signaling Pathways, as well as the VEGF, EGF, FGF, PDGF Receptor Pathways)

Antibodies for Specific Studies (Cell- & Neurobiology, Apoptosis, Signal Transduction, Chemokine & Hormone, Immunology, Cytokine Signaling, Infectious Diseases, etc. ) * Primary & Secondary Antibodies * Gene Regulation Antibodies * M13 Phage, His & GST Antibodies * Monoclonal Antibodies

Monoclonal & Polyclonal Antibodies (Heat Shock, Chemokines, Cytokines, Anti-GST, Anti-Viral, etc.)

Primary & Secondary Antibodies * Conjugates * Heat-Shock & Cellular Signaling Antibodies * Monoclonal & Polyclonal Antibodies