Bioneer AccuTarget siRNAs

Turbo si-Designer: Highly effective in selecting functional siRNAs: 83.8% of the test siRNAs showed >75% knockdown and 38.1% elicited & 90% knockdown...

Successful siRNA experiments in mammalian cultured cells depend upon several factors. Specifically it is important to design and identify effective and specific siRNA sites and to perform efficient and specific delivery of siRNA to the desired target cell types. To facilitate design process, Bioneer, in collaboration with National Genome Information Center (NGIC) has developed Turbo siRNA designer, a proprietary siRNA selection algorithm.

Turbo si-Designer can identify highly effective siRNA target sites with superior success rates. The performance of the Turbo si-Designer was evaluated by designing hundreds of siRNAs and testing their knockdown efficacy by Real-Time PCR analysis. When compared with other web-based design tools, Turbo si-Designer algorithm successfully predicted functional siRNAs at high probability of efficient knockdown. Notably, siRNAs with the low score were mostly nonfunctional, indicating that ineffective siRNAs are efficiently removed by Turbo si-Designer (Fig. 1).

Bioneer manufactures high quality and cost-effective siRNA. Every siRNA is produced in an automated high-throughput RNA production system under clean room conditions and undergo rigorous QC tests.

Features and Benefits

All Bioneer siRNAs are provided as double-stranded siRNA. Each sense siRNA and an antisense RNA are QC'ed by MALDI-TOF. Every annealed siRNA is then QC-tested using PAGE to confirm proper annealing.

Figure 1. MALDI-TOF mass spectrometry analysis of a custom siRNA. All siRNAs are processed by MALDI-TOF mass spectrometry to ensure its quality.

Figure 2. PAGE data of double-stranded custom siRNA. Complementary single-stranded RNA strands were hybridized to form siRNA duplex and analyzed by 15% non-denaturing PAGE.
SS: single-stranded RNA
DS: double-stranded siRNA

Figure 3. Confocal microscopic image of HeLa cells transfected with FITC-labeled negative control siRNA (Cat No.: SN-1021). The fluorescent cells indicate that the HeLa cells were successfully transfected with the siRNA.

Figure 4. Effects of Human GAPDH Positive Control siRNA. HeLa cells were transfected separately with AccuTarget Human GAPDH Positive Control and Negative Control siRNA using Lipofectamine 2000 (Invitrogen) at a final concentration of 100 nM. Total cellular RNA was isolated from cells 24 hours after transfection and subjected to Northern blot and Real-Time PCR analysis. As can be seen from Fig. 1-B, about 3% GAPDH mRNA remained.

Bioneer's core business has always been the manufacture of DNA and RNA in conventional and breakthrough ways. When RNA interference (RNAi) was discovered, Bioneer was there to help provide researchers with the tools they need to accomplish their experiments. Bioneer is currently the market leader in RNAi technology in Asia, and is launching in North America this year. Our RNAi portfolio consists of six areas:

• Predesigned siRNA libraries
• PCR primers for knockdown validation
• Human validated siRNA libraries
• Human siRNA library sets and subsets
• Control siRNAs
• siRNA design and synthesis

All Bioneer's siRNAs are designed using our Turbo si-Designer software. This software identifies highly effective siRNA target sites with remarkably high knockdown rates. Critical parameters including base composition, thermodynamic instability and base preference are all considered in the design algorithm. siRNAs spanning SNP sites are removed and finally, non-specific siRNAs are eliminated following homology searching by BLAST and Smith-Waterman algorithms. The result is an siRNA with extraordinary knockdown efficiency and minimal off-target effects. We are so confident in our software that we have the best guarantee in the industry.

Notice to Purchaser

All siRNA Products: For Research Use Only. Not For Use in Diagnostic Procedures.

Limited License

This product is licensed under European Patents 1144623, 121945 and foreign equivalents from Alnylam Pharmaceuticals, Inc., Cambridge, USA and is provided only for use in academic and commercial research whose purpose is to elucidate gene function, including research to validate potential gene products and pathways for drug discovery and development and to screen non-siRNA based compounds (but excluding the evaluation or characterization of this product as the potential basis for a siRNA-based drug) and not for any other commercial purposes. Information about licenses for commercial use (including discovery and development of siRNA-based drugs) is available from Alnylam Pharmaceuticals, Inc., 300 Third Street, Cambridge, MA 02142, USA.

This product is sold for research use only and is not to be administered to humans or used for medical diagnostics. Buyer acknowledges and agrees that all intellectual property rights in the products (including, without limitation, the siRNA sequences used to create such products) and in any Bioneer technology, intellectual property and know-how used to make or useful for the manufacture or use of the products will at all times remain vested in Bioneer (other than any ownership interest that buyer may have in non-public proprietary target genes supplied by buyer to Bioneer in connection with custom products).

Trademark: AccuTarget is a trademark of Bioneer Corporation.

Product Brochure | FAQs